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Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Amide hydrogen/deuterium exchange (ssHDX-MS) and side-chain photolytic labeling (ssPL-MS) followed by mass spectrometric analysis can be valuable for characterizing lyophilized formulations of protein therapeutics. Labeling followed by suitable proteolytic digestion allows the protein structure and...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:J Vis Exp
Päätekijät: Moorthy, Balakrishnan S., Iyer, Lavanya K., Topp, Elizabeth M.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: MyJove Corporation 2015
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4422116/
https://ncbi.nlm.nih.gov/pubmed/25938927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52503
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