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Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
Amide hydrogen/deuterium exchange (ssHDX-MS) and side-chain photolytic labeling (ssPL-MS) followed by mass spectrometric analysis can be valuable for characterizing lyophilized formulations of protein therapeutics. Labeling followed by suitable proteolytic digestion allows the protein structure and...
Uloženo v:
| Vydáno v: | J Vis Exp |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
MyJove Corporation
2015
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4422116/ https://ncbi.nlm.nih.gov/pubmed/25938927 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52503 |
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