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Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery
The CRISPR/Cas9 system is a robust genome editing technology that works in human cells, animals and plants based on the RNA-programmed DNA cleaving activity of the Cas9 enzyme. Building on previous work (Jinek et al., 2013), we show here that new genetic information can be introduced site-specifical...
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| Publicado en: | eLife |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
eLife Sciences Publications, Ltd
2014
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4383097/ https://ncbi.nlm.nih.gov/pubmed/25497837 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.04766 |
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