Synthetic CRISPR/Cas9 reagents facilitate genome editing and homology directed repair

CRISPR/Cas9 has become a powerful tool for genome editing in zebrafish that permits the rapid generation of loss of function mutations and the knock-in of specific alleles using DNA templates and homology directed repair (HDR). We examined the efficiency of synthetic, chemically modified gRNAs and d...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: DiNapoli, Sara E, Martinez-McFaline, Raul, Gribbin, Caitlin K, Wrighton, Paul J, Balgobin, Courtney A, Nelson, Isabel, Leonard, Abigail, Maskin, Carolyn R, Shwartz, Arkadi, Quenzer, Eleanor D, Mailhiot, Darya, Kao, Clara, McConnell, Sean C, de Jong, Jill L O, Goessling, Wolfram, Houvras, Yariv
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7144937/
https://ncbi.nlm.nih.gov/pubmed/32064511
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa085
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