Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes

We demonstrate editing of post-mitotic neurons in the adult mouse brain following injection of Cas9 ribonucleoprotein (RNP) complexes in the hippocampus, striatum and cortex. Engineered variants of Cas9 with multiple SV40 nuclear localization sequences enabled a ten-fold increase in the efficiency o...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Nat Biotechnol
Päätekijät: Staahl, Brett T., Benekareddy, Madhurima, Coulon-Bainier, Claire, Banfal, Ashwin A., Floor, Stephen N., Sabo, Jennifer K., Urnes, Cole, Acevedo Munares, Gabriela, Ghosh, Anirvan, Doudna, Jennifer A.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2017
Aiheet:
Article
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC6649674/
https://ncbi.nlm.nih.gov/pubmed/28191903
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3806
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