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Genome editing via delivery of Cas9 ribonucleoprotein

The CRISPR-Cas genome editing system is very powerful. The format of the CRISPR reagents and the means of delivery are often important factors in targeting efficiency. Delivery of recombinant Cas9 protein and guide RNA (gRNA) as a preformed ribonucleoprotein (RNP) complex has recently emerged as a p...

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Библиографические подробности
Опубликовано в: :Methods
Главные авторы: DeWitt, Mark A., Corn, Jacob E., Carroll, Dana
Формат: Artigo
Язык:Inglês
Опубликовано: 2017
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC6698184/
https://ncbi.nlm.nih.gov/pubmed/28410976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2017.04.003
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