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Genome editing via delivery of Cas9 ribonucleoprotein
The CRISPR-Cas genome editing system is very powerful. The format of the CRISPR reagents and the means of delivery are often important factors in targeting efficiency. Delivery of recombinant Cas9 protein and guide RNA (gRNA) as a preformed ribonucleoprotein (RNP) complex has recently emerged as a p...
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| Publicado en: | Methods |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6698184/ https://ncbi.nlm.nih.gov/pubmed/28410976 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2017.04.003 |
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