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Genome editing via delivery of Cas9 ribonucleoprotein

The CRISPR-Cas genome editing system is very powerful. The format of the CRISPR reagents and the means of delivery are often important factors in targeting efficiency. Delivery of recombinant Cas9 protein and guide RNA (gRNA) as a preformed ribonucleoprotein (RNP) complex has recently emerged as a p...

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Detalhes bibliográficos
Publicado no:Methods
Main Authors: DeWitt, Mark A., Corn, Jacob E., Carroll, Dana
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6698184/
https://ncbi.nlm.nih.gov/pubmed/28410976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2017.04.003
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