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Identification of a Key Catalytic Intermediate Demonstrates that Nitrogenase is Activated by the Reversible Exchange of N(2) for H(2)

Freeze-quenching nitrogenase during turnover with N(2) traps an S = ½ intermediate that was shown by ENDOR and EPR spectroscopy to contain N(2) or a reduction product bound to the active-site molybdenum-iron cofactor (FeMo-co). To identify this intermediate (termed here EG), we turned to a quench-cr...

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Библиографические подробности
Опубликовано в: :J Am Chem Soc
Главные авторы: Lukoyanov, Dmitriy, Yang, Zhi-Yong, Khadka, Nimesh, Dean, Dennis R., Seefeldt, Lance C., Hoffman, Brian M.
Формат: Artigo
Язык:Inglês
Опубликовано: 2015
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4374740/
https://ncbi.nlm.nih.gov/pubmed/25741750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.5b00103
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