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Reductive Elimination of H(2) Activates Nitrogenase to Reduce the N≡N Triple Bond: Characterization of the E(4)(4H) Janus Intermediate in Wild-Type Enzyme

We have proposed a reductive elimination/oxidative addition (re/oa) mechanism for reduction of N(2) to 2NH(3) by nitrogenase, based on identification of a freeze-trapped intermediate of the α-70(Val→Ile) substituted MoFe protein as the Janus intermediate that stores four reducing equivalents on FeMo...

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Dettagli Bibliografici
Pubblicato in:J Am Chem Soc
Autori principali: Lukoyanov, Dmitriy, Khadka, Nimesh, Yang, Zhi-Yong, Dean, Dennis R., Seefeldt, Lance C., Hoffman, Brian M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5024552/
https://ncbi.nlm.nih.gov/pubmed/27529724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.6b06362
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