Hydrogen–deuterium exchange in imidazole as a tool for studying histidine phosphorylation

Isotope exchange at the histidine C(2) atom of imidazole in D(2)O solution is well known to occur at a significantly slower rate than the exchange of amide protons. Analysis of the kinetics of this isotope-exchange reaction is proposed herein as a method of detecting histidine phosphorylation. This...

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Библиографические подробности
Опубликовано в: :Anal Bioanal Chem
Главные авторы: Cebo, Małgorzata, Kielmas, Martyna, Adamczyk, Justyna, Cebrat, Marek, Szewczuk, Zbigniew, Stefanowicz, Piotr
Формат: Artigo
Язык:Inglês
Опубликовано: Springer Berlin Heidelberg 2014
Предметы:
Research Paper
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4244536/
https://ncbi.nlm.nih.gov/pubmed/25354888
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-014-8218-5
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