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Imidazole C-2 Hydrogen/Deuterium Exchange Reaction at Histidine for Probing Protein Structure and Function with MALDI Mass Spectrometry
We present a mass spectrometric method for analyzing protein structure and function, based on the imidazole C-2 or histidine C(ε(1)) hydrogen/deuterium (H/D) exchange reaction, which is intrinsically second order with respect to the concentrations of the imidazolium cation and OD(−) in D(2)O. The se...
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| Publicado no: | Biochemistry |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4465451/ https://ncbi.nlm.nih.gov/pubmed/24606199 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi401260f |
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