Hydrogen–deuterium exchange in imidazole as a tool for studying histidine phosphorylation

Isotope exchange at the histidine C(2) atom of imidazole in D(2)O solution is well known to occur at a significantly slower rate than the exchange of amide protons. Analysis of the kinetics of this isotope-exchange reaction is proposed herein as a method of detecting histidine phosphorylation. This...

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Publicado en:Anal Bioanal Chem
Autores principales: Cebo, Małgorzata, Kielmas, Martyna, Adamczyk, Justyna, Cebrat, Marek, Szewczuk, Zbigniew, Stefanowicz, Piotr
Formato: Artigo
Lenguaje:Inglês
Publicado: Springer Berlin Heidelberg 2014
Materias:
Research Paper
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4244536/
https://ncbi.nlm.nih.gov/pubmed/25354888
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-014-8218-5
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