Histidine Hydrogen-Deuterium Exchange Mass Spectrometry for Probing the Microenvironment of Histidine Residues in Dihydrofolate Reductase

BACKGROUND: Histidine Hydrogen-Deuterium Exchange Mass Spectrometry (His-HDX-MS) determines the HDX rates at the imidazole C(2)-hydrogen of histidine residues. This method provides not only the HDX rates but also the pK (a) values of histidine imidazole rings. His-HDX-MS was used to probe the microe...

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Detalhes bibliográficos
Main Authors: Miyagi, Masaru, Wan, Qun, Ahmad, Md. Faiz, Gokulrangan, Giridharan, Tomechko, Sara E., Bennett, Brad, Dealwis, Chris
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3040192/
https://ncbi.nlm.nih.gov/pubmed/21359214
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0017055
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