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Facilitated sequence counting and assembly by template mutagenesis

Presently, inferring the long-range structure of the DNA templates is limited by short read lengths. Accurate template counts suffer from distortions occurring during PCR amplification. We explore the utility of introducing random mutations in identical or nearly identical templates to create distin...

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Bibliographic Details
Main Authors: Levy, Dan, Wigler, Michael
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2014
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4217440/
https://ncbi.nlm.nih.gov/pubmed/25313059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1416204111
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