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Coupling mutagenesis and parallel deep sequencing to probe essential residues in a genome or gene
The sequence of a protein determines its function by influencing its folding, structure, and activity. Similarly, the most conserved residues of orthologous and paralogous proteins likely define those most important. The detection of important or essential residues is not always apparent via sequenc...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3587248/ https://ncbi.nlm.nih.gov/pubmed/23401533 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1222538110 |
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