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Mutagenesis-based definitions and probes of residue burial in proteins
Every residue of the 101-aa Escherichia coli toxin CcdB was substituted with Ala, Asp, Glu, Lys, and Arg by using site-directed mutagenesis. The activity of each mutant in vivo was characterized as a function of Controller of Cell Division or Death B protein (CcdB) transcriptional level. The mutatio...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1283427/ https://ncbi.nlm.nih.gov/pubmed/16251276 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0505089102 |
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