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Residue proximity information and protein model discrimination using saturation-suppressor mutagenesis

Identification of residue-residue contacts from primary sequence can be used to guide protein structure prediction. Using Escherichia coli CcdB as the test case, we describe an experimental method termed saturation-suppressor mutagenesis to acquire residue contact information. In this methodology, f...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Sahoo, Anusmita, Khare, Shruti, Devanarayanan, Sivasankar, Jain, Pankaj C., Varadarajan, Raghavan
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4758949/
https://ncbi.nlm.nih.gov/pubmed/26716404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.09532
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