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Residue proximity information and protein model discrimination using saturation-suppressor mutagenesis
Identification of residue-residue contacts from primary sequence can be used to guide protein structure prediction. Using Escherichia coli CcdB as the test case, we describe an experimental method termed saturation-suppressor mutagenesis to acquire residue contact information. In this methodology, f...
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Publicado no: | eLife |
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Main Authors: | , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
eLife Sciences Publications, Ltd
2015
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4758949/ https://ncbi.nlm.nih.gov/pubmed/26716404 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.09532 |
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