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Facilitated sequence counting and assembly by template mutagenesis

Presently, inferring the long-range structure of the DNA templates is limited by short read lengths. Accurate template counts suffer from distortions occurring during PCR amplification. We explore the utility of introducing random mutations in identical or nearly identical templates to create distin...

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Autors principals: Levy, Dan, Wigler, Michael
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4217440/
https://ncbi.nlm.nih.gov/pubmed/25313059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1416204111
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