Partial bisulfite conversion for unique template sequencing

We introduce a new protocol, mutational sequencing or muSeq, which uses sodium bisulfite to randomly deaminate unmethylated cytosines at a fixed and tunable rate. The muSeq protocol marks each initial template molecule with a unique mutation signature that is present in every copy of the template, a...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: Kumar, Vijay, Rosenbaum, Julie, Wang, Zihua, Forcier, Talitha, Ronemus, Michael, Wigler, Michael, Levy, Dan
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2018
Teme:
Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5778454/
https://ncbi.nlm.nih.gov/pubmed/29161423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx1054
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