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Optimization of Critical Hairpin Features Allows miRNA-based Gene Knockdown Upon Single-copy Transduction

Gene knockdown using micro RNA (miRNA)-based vector constructs is likely to become a prominent gene therapy approach. It was the aim of this study to improve the efficiency of gene knockdown through optimizing the structure of miRNA mimics. Knockdown of two target genes was analyzed: CCR5 and green...

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Detalhes bibliográficos
Main Authors: Myburgh, Renier, Cherpin, Ophélie, Schlaepfer, Erika, Rehrauer, Hubert, Speck, Roberto F, Krause, Karl-Heinz, Salmon, Patrick
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4217082/
https://ncbi.nlm.nih.gov/pubmed/25350582
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mtna.2014.58
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