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Knowledge-based design of a biosensor to quantify localized ERK activation in living cells
Investigation of protein activation in living cells is fundamental to understand how proteins are influenced by the full complement of upstream regulators they experience. We describe here the generation of a biosensor based on the Designed Ankyrin Repeat Protein (DARPin) binding scaffold suited for...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4154710/ https://ncbi.nlm.nih.gov/pubmed/23790495 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2013.04.016 |
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