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Redesign of the PAK1 Auto-Inhibitory Domain for enhanced stability and affinity in biosensor applications

The inhibitory switch (IS) domain of p21-activated kinase 1 (PAK1) stabilizes full-length PAK1 in an inactive conformation by binding to the PAK1 kinase domain. Competitive binding of small GTPases to the IS domain disrupts the autoinhibitory interactions and exposes the IS domain binding site on th...

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Detalhes bibliográficos
Main Authors: Jha, Ramesh K., Wu, Yi I., Zawistowski, Jon S, MacNevin, Chris, Hahn, Klaus M., Kuhlman, Brian
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3202338/
https://ncbi.nlm.nih.gov/pubmed/21888918
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2011.08.022
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