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Experimental methods for scanning unnatural amino acid mutagenesis
Site-specific incorporation of unnatural amino acids into proteins in vivo relies on the genetic reassignment of nonsense or quadruplet codons. Here, we describe a general procedure for the random introduction of these codons into open reading frames resulting in protein libraries that are scanned w...
Gorde:
| Egile Nagusiak: | , |
|---|---|
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
2012
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4104607/ https://ncbi.nlm.nih.gov/pubmed/21956563 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-331-8_11 |
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