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Experimental methods for scanning unnatural amino acid mutagenesis
Site-specific incorporation of unnatural amino acids into proteins in vivo relies on the genetic reassignment of nonsense or quadruplet codons. Here, we describe a general procedure for the random introduction of these codons into open reading frames resulting in protein libraries that are scanned w...
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| Main Authors: | , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2012
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4104607/ https://ncbi.nlm.nih.gov/pubmed/21956563 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-331-8_11 |
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