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A method for multi-codon scanning mutagenesis of proteins based on asymmetric transposons
Random mutagenesis followed by selection or screening is a commonly used strategy to improve protein function. Despite many available methods for random mutagenesis, nearly all generate mutations at the nucleotide level. An ideal mutagenesis method would allow for the generation of ‘codon mutations’...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2012
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3258844/ https://ncbi.nlm.nih.gov/pubmed/22184456 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzr060 |
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