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Rational protein sequence diversification by multi-codon scanning mutagenesis
A new method for protein sequence diversification is based on generating random codon mutations to an encoding DNA. This allows for the scanning of user-defined amino acid changes to any protein of interest, and is an alternative to traditional directed evolution strategies. This chapter describes t...
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| Main Authors: | , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2013
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4104598/ https://ncbi.nlm.nih.gov/pubmed/23423900 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-293-3_16 |
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