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Rational protein sequence diversification by multi-codon scanning mutagenesis
A new method for protein sequence diversification is based on generating random codon mutations to an encoding DNA. This allows for the scanning of user-defined amino acid changes to any protein of interest, and is an alternative to traditional directed evolution strategies. This chapter describes t...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4104598/ https://ncbi.nlm.nih.gov/pubmed/23423900 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-293-3_16 |
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