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A method for multi-codon scanning mutagenesis of proteins based on asymmetric transposons

Random mutagenesis followed by selection or screening is a commonly used strategy to improve protein function. Despite many available methods for random mutagenesis, nearly all generate mutations at the nucleotide level. An ideal mutagenesis method would allow for the generation of ‘codon mutations’...

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Hlavní autoři: Liu, Jia, Cropp, T.Ashton
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3258844/
https://ncbi.nlm.nih.gov/pubmed/22184456
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzr060
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