Model based engineering of Pichia pastoris central metabolism enhances recombinant protein production

The production of recombinant proteins is frequently enhanced at the levels of transcription, codon usage, protein folding and secretion. Overproduction of heterologous proteins, however, also directly affects the primary metabolism of the producing cells. By incorporation of the production of a het...

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Autors principals: Nocon, Justyna, Steiger, Matthias G., Pfeffer, Martin, Sohn, Seung Bum, Kim, Tae Yong, Maurer, Michael, Rußmayer, Hannes, Pflügl, Stefan, Ask, Magnus, Haberhauer-Troyer, Christina, Ortmayr, Karin, Hann, Stephan, Koellensperger, Gunda, Gasser, Brigitte, Lee, Sang Yup, Mattanovich, Diethard
Format: Artigo
Idioma:Inglês
Publicat: Academic Press 2014
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4094982/
https://ncbi.nlm.nih.gov/pubmed/24853352
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymben.2014.05.011
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