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A rapid, site-selective and efficient route to the dual modification of DARPins
Designed ankyrin repeat proteins (DARPins) are valuable tools in both biochemistry and medicine. Herein we describe a rapid, simple method for the dual modification of DARPins by introduction of cysteine mutations at specific positions that results in a vast difference in their thiol nucleophilicity...
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| Autori principali: | , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Royal Society of Chemistry
2014
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4091302/ https://ncbi.nlm.nih.gov/pubmed/24687090 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c4cc00053f |
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