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A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification

Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protei...

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Detalhes bibliográficos
Main Authors: Nathani, Ramiz I., Moody, Paul, Chudasama, Vijay, Smith, Mark E. B., Fitzmaurice, Richard J., Caddick, Stephen
Formato: Artigo
Idioma:Inglês
Publicado em: Royal Society of Chemistry 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3985185/
https://ncbi.nlm.nih.gov/pubmed/24741436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c3sc51333e
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