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A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification
Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protei...
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Main Authors: | , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Royal Society of Chemistry
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3985185/ https://ncbi.nlm.nih.gov/pubmed/24741436 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c3sc51333e |
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