Monitoring Protein Misfolding by Site-Specific Labeling of Proteins In Vivo

Incorporating fluorescent amino acids by suppression of the TAG amber codon is a useful tool for site-specific labeling of proteins and visualizing their localization in living cells. Here we use a plasmid encoded orthogonal tRNA/aminoacyl-tRNA synthetase pair to site-specifically label firefly luci...

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Autori principali: Hsieh, Tzung-yang, Nillegoda, Nadinath B., Tyedmers, Jens, Bukau, Bernd, Mogk, Axel, Kramer, Günter
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2014
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4051779/
https://ncbi.nlm.nih.gov/pubmed/24915041
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0099395
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