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Monitoring Protein Misfolding by Site-Specific Labeling of Proteins In Vivo
Incorporating fluorescent amino acids by suppression of the TAG amber codon is a useful tool for site-specific labeling of proteins and visualizing their localization in living cells. Here we use a plasmid encoded orthogonal tRNA/aminoacyl-tRNA synthetase pair to site-specifically label firefly luci...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4051779/ https://ncbi.nlm.nih.gov/pubmed/24915041 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0099395 |
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