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Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
RNA-guided engineered nucleases (RGENs) derived from the prokaryotic adaptive immune system known as CRISPR (clustered, regularly interspaced, short palindromic repeat)/Cas (CRISPR-associated) enable genome editing in human cell lines, animals, and plants, but are limited by off-target effects and u...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Cold Spring Harbor Laboratory Press
2014
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4032847/ https://ncbi.nlm.nih.gov/pubmed/24696461 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.171322.113 |
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