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RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detecti...
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| Publicado no: | G3 (Bethesda) |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4455777/ https://ncbi.nlm.nih.gov/pubmed/24875628 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.114.012179 |
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