DNA targeting specificity of RNA-guided Cas9 nucleases

The Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of singleguide RNAs (sgRNAs) to enable genome editing(1–10). Here, we characterize SpCas9 targeting specificity in human cells to inform the selection of target sites and avoid off-target effects....

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
मुख्य लेखकों: Hsu, Patrick D, Scott, David A, Weinstein, Joshua A, Ran, F Ann, Konermann, Silvana, Agarwala, Vineeta, Li, Yinqing, Fine, Eli J, Wu, Xuebing, Shalem, Ophir, Cradick, Thomas J, Marraffini, Luciano A, Bao, Gang, Zhang, Feng
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: 2013
विषय:
Article
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC3969858/
https://ncbi.nlm.nih.gov/pubmed/23873081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2647
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