DNA targeting specificity of RNA-guided Cas9 nucleases

The Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of singleguide RNAs (sgRNAs) to enable genome editing(1–10). Here, we characterize SpCas9 targeting specificity in human cells to inform the selection of target sites and avoid off-target effects....

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Hsu, Patrick D, Scott, David A, Weinstein, Joshua A, Ran, F Ann, Konermann, Silvana, Agarwala, Vineeta, Li, Yinqing, Fine, Eli J, Wu, Xuebing, Shalem, Ophir, Cradick, Thomas J, Marraffini, Luciano A, Bao, Gang, Zhang, Feng
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3969858/
https://ncbi.nlm.nih.gov/pubmed/23873081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2647
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados