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Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity

Extensively purified DNA gyrase from Escherichia coli is inhibited by nalidixic acid and by novobiocin. The enzyme is composed of two subunits, A and B, which were purified as separate components. Subunit A is the product of the gene controlling sensitivity to nalidixic acid (nalA) because: (i) the...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Higgins, N. Patrick, Peebles, Craig L., Sugino, Akio, Cozzarelli, Nicholas R.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1978
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392422/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/347446/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.4.1773
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