Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity

Extensively purified DNA gyrase from Escherichia coli is inhibited by nalidixic acid and by novobiocin. The enzyme is composed of two subunits, A and B, which were purified as separate components. Subunit A is the product of the gene controlling sensitivity to nalidixic acid (nalA) because: (i) the...

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Bibliografiske detaljer

Udgivet i:	Proc Natl Acad Sci U S A
Principais autores:	Higgins, N. Patrick, Peebles, Craig L., Sugino, Akio, Cozzarelli, Nicholas R.
Format:	Artigo
Sprog:	Inglês
Udgivet:	National Academy of Sciences 1978
Fag:	Biological Sciences: Biochemistry
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392422/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/347446/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.4.1773
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