Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity
Extensively purified DNA gyrase from Escherichia coli is inhibited by nalidixic acid and by novobiocin. The enzyme is composed of two subunits, A and B, which were purified as separate components. Subunit A is the product of the gene controlling sensitivity to nalidixic acid (nalA) because: (i) the...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1978
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392422/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/347446/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.4.1773 |
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