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DNA gyrase: affinity chromatography on novobiocin-Sepharose and catalytic properties.
Novobiocin-Sepharose was prepared by coupling of novobiocin to Epoxy-activated Sepharose 6B and used as an affinity adsorbent. Four novobiocin-binding proteins were isolated from crude extracts of Escherichia coli with molecular weights of 105, 92, 85 and 40 kdal. The two larger proteins were identi...
Enregistré dans:
| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1981
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327377/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6269086/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.15.3589 |
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