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Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity

Extensively purified DNA gyrase from Escherichia coli is inhibited by nalidixic acid and by novobiocin. The enzyme is composed of two subunits, A and B, which were purified as separate components. Subunit A is the product of the gene controlling sensitivity to nalidixic acid (nalA) because: (i) the...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
הוצא לאור ב:Proc Natl Acad Sci U S A
Principais autores: Higgins, N. Patrick, Peebles, Craig L., Sugino, Akio, Cozzarelli, Nicholas R.
פורמט: Artigo
שפה:Inglês
יצא לאור: National Academy of Sciences 1978
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392422/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/347446/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.4.1773
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