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Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity

Extensively purified DNA gyrase from Escherichia coli is inhibited by nalidixic acid and by novobiocin. The enzyme is composed of two subunits, A and B, which were purified as separate components. Subunit A is the product of the gene controlling sensitivity to nalidixic acid (nalA) because: (i) the...

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Bibliografske podrobnosti
Main Authors: Higgins, N. Patrick, Peebles, Craig L., Sugino, Akio, Cozzarelli, Nicholas R.
Format: Artigo
Jezik:Inglês
Izdano: 1978
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC392422/
https://ncbi.nlm.nih.gov/pubmed/347446
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