Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity

Extensively purified DNA gyrase from Escherichia coli is inhibited by nalidixic acid and by novobiocin. The enzyme is composed of two subunits, A and B, which were purified as separate components. Subunit A is the product of the gene controlling sensitivity to nalidixic acid (nalA) because: (i) the...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Higgins, N. Patrick, Peebles, Craig L., Sugino, Akio, Cozzarelli, Nicholas R.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1978
Soggetti:	Biological Sciences: Biochemistry
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392422/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/347446/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.4.1773
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