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Employing the metabolic “branch point effect” to generate an all-or-none, digital-like response in enzymatic outputs and enzyme-based sensors
Here we demonstrate a strategy to convert the graded Michaelis-Menten response typical of unregulated enzymes into a sharp, effectively all-or-none response. We do so using an approach analogous to the “branch point effect”, a mechanism observed in naturally occurring metabolic networks in which two...
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| 主要な著者: | , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2011
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3893712/ https://ncbi.nlm.nih.gov/pubmed/22148353 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac202701c |
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