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Proline Scanning Mutagenesis Reveals a Role for the Flap Endonuclease-1 Helical Cap in Substrate Unpairing

The prototypical 5′-nuclease, flap endonuclease-1 (FEN1), catalyzes the essential removal of single-stranded flaps during DNA replication and repair. FEN1 hydrolyzes a specific phosphodiester bond one nucleotide into double-stranded DNA. This specificity arises from double nucleotide unpairing that...

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Détails bibliographiques
Auteurs principaux: Patel, Nikesh, Exell, Jack C., Jardine, Emma, Ombler, Ben, Finger, L. David, Ciani, Barbara, Grasby, Jane A.
Format: Artigo
Langue:Inglês
Publié: American Society for Biochemistry and Molecular Biology 2013
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3837164/
https://ncbi.nlm.nih.gov/pubmed/24126913
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.509489
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