Increasing cleavage specificity and activity of restriction endonuclease KpnI

Restriction enzyme KpnI is a HNH superfamily endonuclease requiring divalent metal ions for DNA cleavage but not for binding. The active site of KpnI can accommodate metal ions of different atomic radii for DNA cleavage. Although Mg(2+) ion higher than 500 μM mediates promiscuous activity, Ca(2+) su...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Vasu, Kommireddy, Nagamalleswari, Easa, Zahran, Mai, Imhof, Petra, Xu, Shuang-yong, Zhu, Zhenyu, Chan, Siu-Hong, Nagaraja, Valakunja
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2013
Pynciau:
Nucleic Acid Enzymes
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3834813/
https://ncbi.nlm.nih.gov/pubmed/23963701
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt734
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