Increasing cleavage specificity and activity of restriction endonuclease KpnI

Restriction enzyme KpnI is a HNH superfamily endonuclease requiring divalent metal ions for DNA cleavage but not for binding. The active site of KpnI can accommodate metal ions of different atomic radii for DNA cleavage. Although Mg(2+) ion higher than 500 μM mediates promiscuous activity, Ca(2+) su...

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Bibliografiske detaljer
Main Authors: Vasu, Kommireddy, Nagamalleswari, Easa, Zahran, Mai, Imhof, Petra, Xu, Shuang-yong, Zhu, Zhenyu, Chan, Siu-Hong, Nagaraja, Valakunja
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2013
Fag:
Nucleic Acid Enzymes
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3834813/
https://ncbi.nlm.nih.gov/pubmed/23963701
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt734
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