ロード中...

Increasing cleavage specificity and activity of restriction endonuclease KpnI

Restriction enzyme KpnI is a HNH superfamily endonuclease requiring divalent metal ions for DNA cleavage but not for binding. The active site of KpnI can accommodate metal ions of different atomic radii for DNA cleavage. Although Mg(2+) ion higher than 500 μM mediates promiscuous activity, Ca(2+) su...

詳細記述

保存先:
書誌詳細
主要な著者: Vasu, Kommireddy, Nagamalleswari, Easa, Zahran, Mai, Imhof, Petra, Xu, Shuang-yong, Zhu, Zhenyu, Chan, Siu-Hong, Nagaraja, Valakunja
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 2013
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3834813/
https://ncbi.nlm.nih.gov/pubmed/23963701
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt734
タグ: タグ追加
タグなし, このレコードへの初めてのタグを付けませんか!