טוען...
Increasing cleavage specificity and activity of restriction endonuclease KpnI
Restriction enzyme KpnI is a HNH superfamily endonuclease requiring divalent metal ions for DNA cleavage but not for binding. The active site of KpnI can accommodate metal ions of different atomic radii for DNA cleavage. Although Mg(2+) ion higher than 500 μM mediates promiscuous activity, Ca(2+) su...
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| Main Authors: | , , , , , , , |
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| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
2013
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3834813/ https://ncbi.nlm.nih.gov/pubmed/23963701 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt734 |
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