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Increasing cleavage specificity and activity of restriction endonuclease KpnI
Restriction enzyme KpnI is a HNH superfamily endonuclease requiring divalent metal ions for DNA cleavage but not for binding. The active site of KpnI can accommodate metal ions of different atomic radii for DNA cleavage. Although Mg(2+) ion higher than 500 μM mediates promiscuous activity, Ca(2+) su...
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| 主要な著者: | , , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2013
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3834813/ https://ncbi.nlm.nih.gov/pubmed/23963701 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt734 |
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