Increasing cleavage specificity and activity of restriction endonuclease KpnI

Restriction enzyme KpnI is a HNH superfamily endonuclease requiring divalent metal ions for DNA cleavage but not for binding. The active site of KpnI can accommodate metal ions of different atomic radii for DNA cleavage. Although Mg(2+) ion higher than 500 μM mediates promiscuous activity, Ca(2+) su...

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Bibliografski detalji
Glavni autori: Vasu, Kommireddy, Nagamalleswari, Easa, Zahran, Mai, Imhof, Petra, Xu, Shuang-yong, Zhu, Zhenyu, Chan, Siu-Hong, Nagaraja, Valakunja
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2013
Teme:
Nucleic Acid Enzymes
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3834813/
https://ncbi.nlm.nih.gov/pubmed/23963701
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt734
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