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Patch cloning method for multiple site-directed and saturation mutagenesis
BACKGROUND: Various DNA manipulation methods have been developed to prepare mutant genes for protein engineering. However, development of more efficient and convenient method is still demanded. Homologous DNA assembly methods, which do not depend on restriction enzymes, have been used as convenient...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2013
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3829206/ https://ncbi.nlm.nih.gov/pubmed/24164925 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-91 |
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