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Patch cloning method for multiple site-directed and saturation mutagenesis

BACKGROUND: Various DNA manipulation methods have been developed to prepare mutant genes for protein engineering. However, development of more efficient and convenient method is still demanded. Homologous DNA assembly methods, which do not depend on restriction enzymes, have been used as convenient...

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Detalhes bibliográficos
Main Authors: Taniguchi, Naohiro, Nakayama, Sayumi, Kawakami, Takashi, Murakami, Hiroshi
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3829206/
https://ncbi.nlm.nih.gov/pubmed/24164925
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-91
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