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Patch cloning method for multiple site-directed and saturation mutagenesis

BACKGROUND: Various DNA manipulation methods have been developed to prepare mutant genes for protein engineering. However, development of more efficient and convenient method is still demanded. Homologous DNA assembly methods, which do not depend on restriction enzymes, have been used as convenient...

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Main Authors: Taniguchi, Naohiro, Nakayama, Sayumi, Kawakami, Takashi, Murakami, Hiroshi
פורמט: Artigo
שפה:Inglês
יצא לאור: BioMed Central 2013
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3829206/
https://ncbi.nlm.nih.gov/pubmed/24164925
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-91
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