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Complementary annealing mediated by exonuclease: a method for seamless cloning and conditioning site-directed mutagenesis
Traditional cut-paste DNA cloning is often limited by the availability of restriction enzyme sites. Here, we described the complementary annealing mediated by exonuclease (CAME), in which the insert or vector fragment is amplified to carry sequences complementary to the other, and both fragments are...
Gorde:
| Argitaratua izan da: | Biotechnol Biotechnol Equip |
|---|---|
| Egile Nagusiak: | , , , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
Taylor & Francis
2015
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4686905/ https://ncbi.nlm.nih.gov/pubmed/26740790 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/13102818.2014.988094 |
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