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Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris
BACKGROUND: Tagging proteins is a standard method facilitating protein detection, purification or targeting. When tagging a certain protein of interest, it is challenging to predict which tag will give optimal results and will not interfere with protein folding, activity or yields. Ideally, multiple...
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| Publié dans: | Microb Cell Fact |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4501187/ https://ncbi.nlm.nih.gov/pubmed/26169367 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0293-6 |
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