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T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis
The assembly of DNA fragments with homologous arms is becoming popular in routine cloning. For an in vitro assembly reaction, a DNA polymerase is often used either alone for its 3′-5′ exonuclease activity or together with a 5′-3′ exonuclease for its DNA polymerase activity. Here, we present a ‘T5 ex...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6379645/ https://ncbi.nlm.nih.gov/pubmed/30462336 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky1169 |
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